Characterization of 11β-hydroxysteroid dehydrogenase activity in testicular tissue of control and GnRH-immunized boars as a possible regulator of spermatogenesis

被引:14
作者
Claus, R [1 ]
Wagner, A [1 ]
Lambert, T [1 ]
机构
[1] Univ Hohenheim, Inst Tierhaltung & Tierzuchtung, Facggebiet Tierhaltung & Leistungsphysiol 470A, D-7000 Stuttgart, Germany
关键词
11 beta-hydroxysteroid dehydrogenase; boar testis; immunization; cortisol; spermatogenesis;
D O I
10.1055/s-2005-837554
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Glucocorticoids are involved in the regulation of spermatogenesis in the boar testis by initiating apoptosis in early stages of germ cell development. Because cortisol activity is modulated by the 11 beta-hydroxysteroid dehydrogenase system (11 beta-HSD), the present study determined both 11 beta-activating (reductive) and inactivating (oxidative) enzyme activities in testicular tissue preparations of control boars (n = 5), GnRH-immunized boars (n = 5), and immunized, estradiol-infused boars (n = 6) by radioenzyme assay based on the conversion of tritiated cortisol to cortisone in the presence of NAD(+) (inactivation) or tritiated cortisone to cortisol in the presence of NADPH (activation). The presence of both isoforms, 11 beta-HSD 1 and 11 beta-HSD 2, was confirmed by RTPCR in testicular tissue of control boars. Additionally, cortisol, testosterone, estradiol, and LH were determined in blood plasma sampled twice before killing. Immunization led to a drop of LH from 4.3 +/- 0.3 pmol/L to 1.0 +/- 0.3 pmol/L (testosterone: 11.63 +/- 0.83 nmol/L vs. 0.28 +/- 0.07 nmol/L; 17 beta-estradiol: 512.61 +/- 47.60 pmol/L vs. 77.05 +/- 14.00 pmol/L). Low 11 beta-HSD reductive activity was found in boars (1 pmol steroid x min(-1) x mg(-1)). It decreased to trace amounts in immunized boars (0.07 pmol steroid x min(-1) x mg(-1)). Oxidative activity was found in boars with 10.19 +/- 2.28 pmol steroid x min(-1) x mg-1 protein. Immunization led to a sharp decrease (0.08 +/- 0.03 pmol x min(-1) x mg(-1)). Infusion of 17 beta-estradiol significantly elevated peripheral estradiol concentrations to 752.07 +/- 24.19 pmol/L which still is a physiological concentration in this species. The infusion led to a minimal reductive activity (0.04 pmol steroid x min(-1) x mg(-1)), but led to a 6-fold rise of the 11 beta-HSD oxidative activity to 0.47 +/- 0.14 pmol x min(-1) x mg(-1) compared to immunized boars. It is concluded that the 11 beta-HSD system is involved in the regulation of cortisol activity in the testis and thus in the regulation of spermatogenesis.
引用
收藏
页码:262 / 267
页数:6
相关论文
共 49 条
[1]   EXPRESSION OF 11-BETA-HYDROXYSTEROID DEHYDROGENASE USING RECOMBINANT VACCINIA VIRUS [J].
AGARWAL, AK ;
TUSIELUNA, MT ;
MONDER, C ;
WHITE, PC .
MOLECULAR ENDOCRINOLOGY, 1990, 4 (12) :1827-1832
[2]  
ALBISTON AL, 1994, MOL CELL ENDOCRINOL, V105, P11
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   HUMAN PLACENTAL 11-BETA-HYDROXYSTEROID DEHYDROGENASE - EVIDENCE FOR AND PARTIAL-PURIFICATION OF A DISTINCT NAD-DEPENDENT ISOFORM [J].
BROWN, RW ;
CHAPMAN, KE ;
EDWARDS, CRW ;
SECKL, JR .
ENDOCRINOLOGY, 1993, 132 (06) :2614-2621
[5]   PHOTOPERIODICITY AND CIRCANNUAL LEVELS OF LH, FSH, AND TESTOSTERONE IN NORMAL AND CASTRATED MALE, WHITE-TAILED DEER [J].
BUBENIK, GA ;
MORRIS, JM ;
SCHAMS, D ;
CLAUS, A .
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1982, 60 (06) :788-793
[6]   Androgen and glucocorticoid receptor heterodimer formation - A possible mechanism for mutual inhibition of transcriptional activity [J].
Chen, SY ;
Wang, J ;
Yu, GQ ;
Liu, WH ;
Pearce, D .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (22) :14087-14092
[7]   CONTRIBUTION OF INDIVIDUAL COMPARTMENTS OF THE GENITAL-TRACT TO ESTROGEN AND TESTOSTERONE CONCENTRATIONS IN EJACULATES OF THE BOAR [J].
CLAUS, R ;
SCHOPPER, D ;
HOANGVU, C .
ACTA ENDOCRINOLOGICA, 1985, 109 (02) :281-288
[8]   EFFECT OF MALE ODOR ON LH-SECRETION AND THE INDUCTION OF OVULATION IN SEASONALLY ANESTROUS GOATS [J].
CLAUS, R ;
OVER, R ;
DEHNHARD, M .
ANIMAL REPRODUCTION SCIENCE, 1990, 22 (01) :27-38
[9]   ESTROGENS, COMPARED TO OTHER STEROIDS OF TESTICULAR ORIGIN, IN BLOOD-PLASMA OF BOARS [J].
CLAUS, R ;
HOFFMANN, B .
ACTA ENDOCRINOLOGICA, 1980, 94 (03) :404-411
[10]   Relationships between IGF-I, cortisol, and osteocalcin in peripheral plasma of growing pigs [J].
Claus, R ;
Weiler, U .
EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES, 1996, 104 (04) :344-349