Characterization of a novel pectate lyase, Pel10A, from Pseudomonas cellulosa

被引:10
作者
Charnock, SJ
Brown, IE
Turkenburg, JP
Black, GW
Davies, GJ
机构
[1] Univ Sunderland, Sch Sci, Sunderland SR1 3SD, Tyne & Wear, England
[2] Univ York, Dept Chem, Struct Biol Lab, York YO10 5DD, N Yorkshire, England
来源
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2001年 / 57卷
关键词
D O I
10.1107/S0907444901007491
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Biological recycling of plant material is essential for biosphere maintenance. This perpetual task involves a complex array of enzymes, including extracellular polysaccharide hydrolases and lyases. Whilst much is known about the structure and function of the hydrolases, relatively little is known about the structures and mechanisms of the corresponding lyases. To this end, crystals of the catalytic module of a novel family 10 pectate lyase, Pel10A from Pseudomonas cellulosa, were obtained using polyethylene glycol 2000 monomethylether as a precipitant. They belong to space group P2(1), with unit-cell parameters a = 47.7, b = 106.1, c = 55.4 Angstrom, beta = 92.0 degrees, and have two molecules in the asymmetric unit. The crystals diffract beyond 1.5 Angstrom using synchrotron radiation.
引用
收藏
页码:1141 / 1143
页数:3
相关论文
共 11 条
[1]   EXTRACELLULAR ENZYMES AND PATHOGENESIS OF SOFT-ROT ERWINIA [J].
BARRAS, F ;
VANGIJSEGEM, F ;
CHATTERJEE, AK .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1994, 32 :201-234
[2]   Azospirillum irakense produces a novel type of pectate lyase [J].
Bekri, MA ;
Desair, J ;
Keijers, V ;
Proost, P ;
Searle-Van Leeuwen, M ;
Vanderleyden, J ;
Vande Broek, A .
JOURNAL OF BACTERIOLOGY, 1999, 181 (08) :2440-2447
[3]  
Brown IE, 2001, BIOCHEM J, V355, P155, DOI 10.1042/0264-6021:3550155
[4]   Clear strategy screens for macromolecular crystallization [J].
Brzozowski, AM ;
Walton, J .
JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2001, 34 :97-101
[5]  
Coutinho PM, 1999, ROY SOC CH, P3
[6]  
HENRSSAT B, 2001, IN PRESS
[7]   NUCLEOTIDE-SEQUENCE AND EXPRESSION OF A NOVEL PECTATE LYASE GENE (PEL-3) AND A CLOSELY LINKED ENDOPOLYGALACTURONASE GENE (PEH-1) OF ERWINIA-CAROTOVORA SUBSP CAROTOVORA-71 [J].
LIU, Y ;
CHATTERJEE, A ;
CHATTERJEE, AK .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (07) :2545-2552
[8]   SOLVENT CONTENT OF PROTEIN CRYSTALS [J].
MATTHEWS, BW .
JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) :491-+
[9]   Processing of X-ray diffraction data collected in oscillation mode [J].
Otwinowski, Z ;
Minor, W .
MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 :307-326
[10]   Nucleotide and amino-acid sequences of a new-type pectate lyase from an alkaliphilic strain of Bacillus [J].
Sawada, K ;
Ogawa, A ;
Ozawa, T ;
Sumitomo, N ;
Hatada, Y ;
Kobayashi, T ;
Ito, S .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (05) :1510-1515