Remdesivir, lopinavir, emetine, and homoharringtonine inhibit SARS-CoV-2 replication in vitro

被引：654

作者：

Choy, Ka-Tim ^{[1
]}

Wong, Alvina Yin-Lam ^{[1
]}

Kaewpreedee, Prathanporn ^{[1
]}

Sia, Sin Fun ^{[1
]}

Chen, Dongdong ^{[1
]}

Hui, Kenrie Pui Yan ^{[1
]}

Chu, Daniel Ka Wing ^{[1
]}

Chan, Michael Chi Wai ^{[1
]}

Cheung, Peter Pak-Hang ^{[2
]}

Huang, Xuhui ^{[2
]}

Peiris, Malik ^{[1
]}

Yen, Hui-Ling ^{[1
]}

机构：

[1] Univ Hong Kong, LKS Fac Med, Sch Publ Hlth, Hong Kong, Peoples R China

[2] Hong Kong Univ Sci & Technol, Dept Chem, Hong Kong, Peoples R China

来源：

ANTIVIRAL RESEARCH | 2020年 / 178卷

基金：

美国国家卫生研究院;

关键词：

COVID-19; Remdesivir; Lopinavir; Ritonavir; Emetine; Homoharringtonine;

D O I：

10.1016/j.antiviral.2020.104786

中图分类号：

R9 [药学];

学科分类号：

100702 [药剂学];

摘要：

An escalating pandemic by the novel SARS-CoV-2 virus is impacting global health and effective therapeutic options are urgently needed. We evaluated the in vitro antiviral effect of compounds that were previously reported to inhibit coronavirus replication and compounds that are currently under evaluation in clinical trials for SARS-CoV-2 patients. We report the antiviral effect of remdesivir, lopinavir, homorringtonine, and emetine against SARS-CoV-2 virus in Vero E6 cells with the estimated 50% effective concentration at 23.15 mu M, 26.63 mu M, 2.55 mu M and 0.46 mu M, respectively. Ribavirin or favipiravir that are currently evaluated under clinical trials showed no inhibition at 100 mu M. Synergy between remdesivir and emetine was observed, and remdesivir at 6.25 mu M in combination with emetine at 0.195 mu M may achieve 64.9% inhibition in viral yield. Combinational therapy may help to reduce the effective concentration of compounds below the therapeutic plasma concentrations and provide better clinical benefits.

引用

页数：5

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