Genetic and Biochemical Characterization of a Pathway for the Degradation of 2-Aminoethylphosphonate in Sinorhizobium meliloti 1021

被引：35

作者：

Borisova, Svetlana A. ^{[1
,4
]}

Christman, Harry D. ^{[2
]}

Metcalf, M. E. Mourey ^{[2
]}

Zulkepli, Nurul A. ^{[3
]}

Zhang, Jun Kai ^{[2
]}

van der Donk, Wilfred A. ^{[1
,3
,4
]}

Metcalf, William W. ^{[1
,2
]}

机构：

[1] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA

[3] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[4] Univ Illinois, Howard Hughes Med Inst, Urbana, IL 61801 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2011年 / 286卷 / 25期

基金：

美国国家卫生研究院;

关键词：

PSEUDOMONAS-FLUORESCENS; 23F; BOND-CLEAVAGE ENZYME; ALDEHYDE DEHYDROGENASE; PHOSPHONOPYRUVATE HYDROLASE; SP PAL2; SUPERFAMILY; MUTAGENESIS; BACTERIA; SEQUENCE; BIODEGRADATION;

D O I：

10.1074/jbc.M111.237735

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A variety of microorganisms have the ability to use phosphonic acids as sole sources of phosphorus. Here, a novel pathway for degradation of 2-aminoethylphosphonate in the bacterium Sinorhizobium meliloti 1021 is proposed based on the analysis of the genome sequence. Gene deletion experiments confirmed the involvement of the locus containing phnW, phnA, and phnY genes in the conversion of 2-aminoethylphosphonate to inorganic phosphate. Biochemical studies of the recombinant PhnY and PhnA proteins verified their roles as phosphonoacetaldehyde dehydrogenase and phosphonoacetate hydrolase, respectively. This pathway is likely not limited to S. meliloti as suggested by the presence of homologous gene clusters in other bacterial genomes.

引用

页码：22283 / 22290

页数：8

共 36 条

[1]

BALDOMA L, 1987, J BIOL CHEM, V262, P13991

[2] Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid [J].

Barnett, MJ ;

Fisher, RF ;

Jones, T ;

Komp, C ;

Abola, AP ;

Barloy-Hubler, F ;

Bowser, L ;

Capela, D ;

Galibert, F ;

Gouzy, J ;

Gurjal, M ;

Hong, A ;

Huizar, L ;

Hyman, RW ;

Kahn, D ;

Kahn, ML ;

Kalman, S ;

Keating, DH ;

Palm, C ;

Peck, MC ;

Surzycki, R ;

Wells, DH ;

Yeh, KC ;

Davis, RW ;

Federspiel, NA ;

Long, SR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) :9883-9888

[3] Biosynthesis of Rhizocticins, Antifungal Phosphonate Oligopeptides Produced by Bacillus subtilis ATCC6633 [J].

Borisova, Svetlana A. ;

Circello, Benjamin T. ;

Zhang, Jun Kai ;

van der Donk, Wilfred A. ;

Metcalf, William W. .

CHEMISTRY & BIOLOGY, 2010, 17 (01) :28-37

[4] Evolutionary genomics of the HAD superfamily: Understanding the structural adaptations and catalytic diversity in a superfamily of phosphoesterases and allied enzymes [J].

Burroughs, A. Maxwell ;

Allen, Karen N. ;

Dunaway-Mariano, Debra ;

Aravind, L. .

JOURNAL OF MOLECULAR BIOLOGY, 2006, 361 (05) :1003-1034

[5] Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021 [J].

Capela, D ;

Barloy-Hubler, F ;

Gouzy, J ;

Bothe, G ;

Ampe, F ;

Batut, J ;

Boistard, P ;

Becker, A ;

Boutry, M ;

Cadieu, E ;

Dréano, S ;

Gloux, S ;

Godrie, T ;

Goffeau, A ;

Kahn, D ;

Kiss, E ;

Lelaure, V ;

Masuy, D ;

Pohl, T ;

Portetelle, D ;

Pühler, A ;

Purnelle, B ;

Ramsperger, U ;

Renard, C ;

Thébault, P ;

Vandenbol, M ;

Weidner, S ;

Galibert, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) :9877-9882

[6] Structure and kinetics of phosphonopyruvate hydrolase from Voriovorax sp Pal2:: New insight into the divergence of catalysis within the PEP mutase/isocitrate lyase superfamily [J].

Chen, Celia C. H. ;

Han, Ying ;

Niu, Weiling ;

Kulakova, Anna N. ;

Howard, Andrew ;

Quinn, John P. ;

Dunaway-Mariano, Debra ;

Herzberg, Osnat .

BIOCHEMISTRY, 2006, 45 (38) :11491-11504

[7] Marine organic phosphorus cycling: Novel insights from nuclear magnetic resonance [J].

Clark, LL ;

Ingall, ED ;

Benner, R .

AMERICAN JOURNAL OF SCIENCE, 1999, 299 (7-9) :724-737

[8] STRUCTURE AND MECHANISM OF ALKALINE-PHOSPHATASE [J].

COLEMAN, JE .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1992, 21 :441-483

[9] The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti [J].

Finan, TM ;

Weidner, S ;

Wong, K ;

Buhrmester, J ;

Chain, P ;

Vorhölter, FJ ;

Hernandez-Lucas, I ;

Becker, A ;

Cowie, A ;

Gouzy, J ;

Golding, B ;

Pühler, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) :9889-9894

[10] RADICAL-BASED DEPHOSPHORYLATION AND ORGANOPHOSPHONATE BIODEGRADATION [J].

FROST, JW ;

LOO, S ;

CORDEIRO, ML ;

LI, D .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1987, 109 (07) :2166-2171

← 1 2 3 4 →