Generation of phytate-free seeds in Arabidopsis through disruption of inositol polyphosphate kinases

被引:225
作者
Stevenson-Paulik, J
Bastidas, RJ
Chiou, ST
Frye, RA
York, JD [1 ]
机构
[1] Duke Univ, Med Ctr, Howard Hughes Med Inst, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA
[2] Pittsburgh Vet Adm Med Ctr, Dept Pathol, Pittsburgh, PA 15240 USA
关键词
genetically engineered crops; low phytate; signal transduction; phosphate sensing;
D O I
10.1073/pnas.0504172102
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Phytate (inositol hexalkisphosphate, IN is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Here, we report a requirement for inositol polyphosphate kinases, AtIPK1 and AtIPK2 beta, for the later steps of phytate synthesis in Arabidopsis thaliana. Coincident disruption of these kinases nearly ablates seed phytate without accumulation of phytate precursors, increases seed-free phosphate by 10-fold, and has normal seed yield. Additionally, we find a requirement for inositol tetrakisphosphate (IP4)/inositol pentakisphosphate ON 2-kinase activity in phosphate sensing and root hair elongation. Our results define a commercially viable strategy for the genetic engineering of phytate-free grain and provide insights into the role of inositol polyphosphate kinases in phosphate signaling biology.
引用
收藏
页码:12612 / 12617
页数:6
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