The Tetrahymena chaperonin subunit CCT eta gene is coexpressed with CCT gamma gene during cilia biogenesis and cell sexual reproduction

被引:25
作者
Cyrne, L
Guerreiro, P
Cardoso, AC
RodriguesPousada, C
Soares, H
机构
[1] INST GULBENKIAN CIENCIAS, GENET MOLEC LAB, P-2781 OEIRAS, PORTUGAL
[2] UNIV LISBON, FAC CIENCIAS LISBOA, DEPT QUIM & BIOQUIM, P-1699 LISBON, PORTUGAL
来源
FEBS LETTERS | 1996年 / 383卷 / 03期
关键词
CCT eta-chaperonin gene; ciliated protozoan; gene structure; gene expression;
D O I
10.1016/0014-5793(96)00240-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report here the cloning and the characterization of the T. pyriformis CCT eta gene (TpCCT eta) and also a partial sequence of the corresponding T. thermophila gene (TtCCT eta). The TpCCT eta gene encodes a protein sharing a 60.3% identity with the mouse CCT eta. We have studied the expression of these genes in Tetrahymena exponentially growing cells, cells regenerating their cilia for different periods and during different stages of the cell sexual reproduction. These genes have similar patterns of expression to those of the previously identified TpCCT gamma gene. Indeed, the Tetrahymena CCT eta and CCT gamma genes are up-regulated at 60-120 min of cilia recovery, and in conjugation when vegetative growth was resumed and cell division took place. Our results seem to indicate that both CCT subunits play an important role in the biogenesis of the newly synthesized cilia of Tetrahymena and during its cell division.
引用
收藏
页码:277 / 283
页数:7
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