Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation

被引：21

作者：

Brittle, Amy L.

Nanba, Yasuaki

Ito, Takashi

Ohkura, Hiroyuki ^{[1
]}

机构：

[1] Univ Edinburgh, Sch Biol Sci, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland

[2] Univ Edinburgh, Sch Biol Sci, Inst Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland

[3] Nagasaki Univ, Sch Med, Dept Biochem, Nagasaki 8528523, Japan

来源：

EXPERIMENTAL CELL RESEARCH | 2007年 / 313卷 / 13期

基金：

英国惠康基金;

关键词：

histone; kinase; NHK-1; Aurora; Polo; cyclin; centromere; Drosophila;

D O I：

10.1016/j.yexcr.2007.04.038

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The spatial and temporal control of histone modifications is crucial for precise regulation of chromatin structure and function. Here we report that phosphorylation of H2A at threonine 119 (T119) is enriched at centromere regions in Drosophila mitosis. We found that the Aurora B kinase complex is essential for this phosphorylation at centromeres, while Polo kinase is required to down-regulate H2A phosphorylation on chromosome arms in mitosis. Cyclin B degradation triggers loss of centromeric H2A phosphorylation at anaphase onset. Epistasis analysis indicated that Polo functions upstream of the H2A kinase NHK-1 but parallel to Aurora B. Therefore, multiple mitotic kinases work together to specify the spatial and temporal pattern of H2A T119 phosphorylation. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：2780 / 2785

页数：6

共 23 条

[1] Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation [J].