Paeoniflorin ameliorates acute necrotizing pancreatitis and pancreatitis-induced acute renal injury

被引：81

作者：

Wang, Peng ^{[1
]}

Wang, Weixing ^{[1
]}

Shi, Qiao ^{[1
]}

Zhao, Liang ^{[1
]}

Mei, Fangchao ^{[1
]}

Li, Chen ^{[1
]}

Zuo, Teng ^{[1
]}

He, Xiaobo ^{[1
]}

机构：

[1] Wuhan Univ, Renmin Hosp, Dept Hepatobiliary & Laparoscop Surg, 99 Ziyang Rd, Wuhan 430060, Hubei, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2016年 / 14卷 / 02期

基金：

中国国家自然科学基金;

关键词：

paeoniflorin; acute necrotizing pancreatitis; acute renal injury; apoptosis; nuclear factor-kappa B; p38 mitogen-activated protein kinases; NF-KAPPA-B; ACTIVATED PROTEIN-KINASE; ACUTE KIDNEY INJURY; INFLAMMATORY MEDIATORS; TNF-ALPHA; APOPTOSIS; RATS; INHIBITION; EXPRESSION; RECEPTOR;

D O I：

10.3892/mmr.2016.5351

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Acute renal injury caused by acute necrotizing pancreatitis (ANP) is a common complication that is associated with a high rate of mortality. Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti-inflammatory, anticancer, analgesic and immunomodulatory effects. The present study detected the potential treatment effects of paeoniflorin on acute renal injury induced by ANP in a rat model. The optimal dose of paeoniflorin for preventing acute renal injury induced by ANP was determined. Then, the possible protective mechanism of paeoniflorin was investigated. The serum levels of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta and IL-6 were measured with enzyme-linked immunosorbent assay kits. Renal inflammation and apoptosis were measured by immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay. The expression of nitric oxide in kidney tissues was also evaluated. The p38 mitogen-activated protein kinases (MAPKs) were measured by western blotting. The results shown that paeoniflorin may ameliorate acute renal injury following ANP in rats by inhibiting inflammatory responses and renal cell apoptosis. These effects may be associated with the p38MAPK and nuclear factor-kappa B signal pathway.

引用

页码：1123 / 1131

页数：9

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