Spectral characterization of NAD(P)H fluorescence in intact isolated chloroplasts and leaves: Effect of chlorophyll concentration on reabsorption of blue-green fluorescence

被引：19

作者：

Cerovic, ZG ^{[1
]}

Langrand, E ^{[1
]}

Latouche, G ^{[1
]}

Morales, F ^{[1
]}

Moya, I ^{[1
]}

机构：

[1] Univ Paris 11, Lab Utilisat Rayonnement Electromagnet, F-91405 Orsay, France

来源：

PHOTOSYNTHESIS RESEARCH | 1998年 / 56卷 / 03期

关键词：

blue-green fluorescence (BGF); intact isolated chloroplasts; Pisum sativum; photosynthesis; pyridine nucleotides; Spinacia oleracea L;

D O I：

10.1023/A:1006081209887

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In the present communication we report a spectral analysis of the blue-green fluorescence related to changes in NAD(P) redox state in chloroplasts and leaves. To assess the contribution of reabsorption and the inner filter effect, we compared transmission and fluorescence at different chloroplast concentrations, and showed that reabsorption by the photosynthetic pigments (chlorophylls and carotenoids) was at the origin of the two peaks in the emission spectrum in vivo. The absence of potential green-emitting fluorophores in chloroplasts was determined by measuring variable and time-resolved fluorescence at different wavelengths. We defined the conditions which optimize the UV-excited blue-green fluorescence signal dependent on NAD(P)H, and we present an example of monitoring of NAD(P)H fluorescence in intact leaves.

引用

页码：291 / 301

页数：11

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