Determination of the major constituents in fruit of Arctium lappa L. by matrix solid-phase dispersion extraction coupled with HPLC separation and fluorescence detection

被引：57

作者：

Liu, He ^{[1
]}

Zhang, Yupu ^{[1
]}

Sun, Yantao ^{[1
]}

Wang, Xue ^{[1
]}

Zhai, Yujuan ^{[1
]}

Sun, Ye ^{[1
]}

Sun, Shuo ^{[1
]}

Yu, Aimin ^{[1
]}

Zhang, Hanqi ^{[1
]}

Wang, Yinghua ^{[1
]}

机构：

[1] Jilin Univ, Coll Chem, Changchun 130012, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2010年 / 878卷 / 28期

关键词：

Arctiin; Arctigenin; Matrix solid-phase dispersion extraction (MSPD); High-performance liquid chromatography (HPLC); Fluorescence detection; PERFORMANCE LIQUID-CHROMATOGRAPHY; IDENTIFICATION; ARCTIGENIN; FRUCTUS; LEAVES;

D O I：

10.1016/j.jchromb.2010.08.007

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

The arctiin and arctigenin in the fruit of Arctium lappa L were extracted by matrix solid-phase dispersion (MSPD) and determined by high-performance liquid chromatography (HPLC) with fluorescence detection. The experimental conditions for the MSPD were optimized. Silica gel was selected as dispersion adsorbent and methanol as elution solvent. The calibration curve showed good relationship (r>0.9998) in the concentration range of 0.010-5.0 mu g mL(-1) for arctiin and 0.025-7.5 mu g mL(-1) for arctigenin. The recoveries were between 74.4% and 100%. The proposed method consumed less sample, time and solvent compared with conventional methods, including ultrasonic and Soxhlet extraction. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：2707 / 2711

页数：5

共 22 条

[1]

ANIBAS AS, 2007, TALANTA, V71, P430

[2]

Recent developments in matrix solid-phase dispersion extraction [J].