Purification and partial characterization of (6-4) photoproduct DNA photolyase from Xenopus laevis

被引：39

作者：

Kim, ST

Malhotra, K

Taylor, JS

Sancar, A

机构：

[1] UNIV N CAROLINA,SCH MED,DEPT BIOCHEM & BIOPHYS,CHAPEL HILL,NC 27599

[2] WASHINGTON UNIV,DEPT CHEM,ST LOUIS,MO 63130

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1996年 / 63卷 / 03期

关键词：

D O I：

10.1111/j.1751-1097.1996.tb03028.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The (6-4) photoproduct DNA photolyase was detected in two vertebrate animals Crotalus atr ox (rattlesnake) and Xenopus laevis (South African clawed toad). The enzyme was extensively purified from X. laevis and characterized. The highly purified enzyme is fluorescent with an excitation maximum at 420-440 nm and emission maximum at 460-480 nm. The photorepair action spectrum matches the fluoresoence excitation spectrum with a 430 nm maximum.

引用

页码：292 / 295

页数：4

共 19 条

[1] COVALENT ATTACHMENT OF DNA TO AGAROSE - IMPROVED SYNTHESIS AND USE IN AFFINITY CHROMATOGRAPHY [J].