Thyroxine and reserpine-induced changes in metabolic profiles of rat urine and the therapeutic effect of Liu Wei Di Huang Wan detected by UPLC-HDMS

被引：152

作者：

Wang, Ping ^{[1
]}

Sun, Hui ^{[1
]}

Lv, Haitao ^{[1
]}

Sun, Wenjun ^{[1
]}

Yuan, Ye ^{[1
]}

Han, Ying ^{[1
]}

Wang, Dongwu ^{[1
]}

Zhang, Aihua ^{[1
]}

Wang, Xijun ^{[1
]}

机构：

[1] Heilongjiang Univ Chinese Med, Natl TCM Key Lab Serum Pharmacochem, Harbin 150040, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2010年 / 53卷 / 03期

关键词：

Metabonomics; UPLC-HDMS; Liu Wei Di Huang Wan; Kidney yin deficiency; L-LYSINE; PLASMA;

D O I：

10.1016/j.jpba.2010.04.032

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

The promise of metabonomics. a new "omics" technique, to validate Chinese medicines and the compatibility of Chinese formulas has been appreciated. The present study was undertaken to explore the excretion pattern of low molecular mass metabolites in the male Wistar-derived rat model of kidney yin deficiency induced with thyroxine and reserpine as well as the therapeutic effect of Liu Wei Di Huang Wan (LW) and its separated prescriptions, a classic traditional Chinese medicine formula for treating kidney yin deficiency in China The study utilized ultra-performance liquid chromatography/electrospray ionization synapt high definition mass spectrometry (UPLC/ESI-SYNAPT-HDMS) in both negative and positive electrospray ionization (ESI) At the same time, blood biochemistry was examined to identify specific changes in the kidney yin deficiency Distinct changes in the pattern of metabolites, as a result of daily administration of thyroxine and reserpine, were observed by UPLC-HDMS combined with a principal component analysis (PCA). The changes in metabolic profiling were restored to their baseline values after treatment with LW according to the PCA score plots Altogether, the current metabonomic approach based on UPLC-HDMS and orthogonal projection to latent structures discriminate analysis (OPLS-DA) indicated 20 ions (14 in the negative mode, 8 in the positive mode, and 2 in both) as "differentiating metabolites" Crown Copyright (C) 2010 Published by Elsevier B V All rights reserved

引用

页码：631 / 645

页数：15

共 29 条

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