The HIV Env-mediated fusion reaction

被引:326
作者
Gallo, SA [1 ]
Finnegan, CM [1 ]
Viard, M [1 ]
Raviv, Y [1 ]
Dimitrov, A [1 ]
Rawat, SS [1 ]
Puri, A [1 ]
Durell, S [1 ]
Blumenthal, R [1 ]
机构
[1] NCI, Lab Expt & Computat Biol, Ctr Canc Res, NIH, Frederick, MD 21702 USA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2003年 / 1614卷 / 01期
关键词
HIV; fusion; Env; SIV; virus; CD4; gp120; gp41; CXCR4; CCR5; six-helix bundle;
D O I
10.1016/S0005-2736(03)00161-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The current general model of HIV viral entry involves the binding of the trimeric viral envelope glycoprotein gp120/gp41 to cell surface receptor CD4 and chemokine co-receptor CXCR4 or CCR5, which triggers conformational changes in the envelope proteins. Gp 120 then dissociates from gp41, allowing for the fusion peptide to be inserted into the target membrane and the pre-hairpin configuration of the ectodomain to form. The C-terminal heptad repeat region and the leucine/isoleucine zipper region then form the thermostable six-helix coiled-coil, which drives the membrane merger and eventual fusion. This model needs updating, as there has been a wealth of data produced in the last few years concerning HIV entry, including target cell dependencies, fusion kinetic data, and conformational intermediates. A more complete model must include the involvement of membrane microdomains, actin polymerization, glycosphingolipids, and possibly CD4 and chemokine signaling in entry. In addition, kinetic experiments involving the addition of fusion inhibitors have revealed some of the rate-limiting steps in this process, adding a temporal component to the model. A review of these data that may require an updated version of the original model is presented here. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:36 / 50
页数:15
相关论文
共 171 条
  • [1] The B-oligomer of pertussis toxin inhibits human immunodeficiency virus type 1 replication at multiple stages
    Alfano, M
    Pushkarsky, T
    Poli, G
    Bukrinsky, M
    [J]. JOURNAL OF VIROLOGY, 2000, 74 (18) : 8767 - 8770
  • [2] CC CKRS: A RANTES, MIP-1 alpha, MIP-1 beta receptor as a fusion cofactor for macrophage-tropic HIV-1
    Alkhatib, G
    Combadiere, C
    Broder, CC
    Feng, Y
    Kennedy, PE
    Murphy, PM
    Berger, EA
    [J]. SCIENCE, 1996, 272 (5270) : 1955 - 1958
  • [3] The transmembrane domain of influenza hemagglutinin exhibits a stringent length requirement to support the hemifusion to fusion transition
    Armstrong, RT
    Kushnir, AS
    White, JM
    [J]. JOURNAL OF CELL BIOLOGY, 2000, 151 (02) : 425 - 437
  • [4] Truncation of the COOH-terminal region of the paramyxovirus SV5 fusion protein leads to hemifusion but not complete fusion
    Bagai, S
    Lamb, RA
    [J]. JOURNAL OF CELL BIOLOGY, 1996, 135 (01) : 73 - 84
  • [5] Membrane fusion mediated by coiled coils: A hypothesis
    Bentz, J
    [J]. BIOPHYSICAL JOURNAL, 2000, 78 (02) : 886 - 900
  • [6] A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus type 1 strains
    Berson, JF
    Long, D
    Doranz, BJ
    Rucker, J
    Jirik, FR
    Doms, RW
    [J]. JOURNAL OF VIROLOGY, 1996, 70 (09) : 6288 - 6295
  • [7] Membrane fusion
    Blumenthal, R
    Clague, MJ
    Durell, SR
    Epand, RM
    [J]. CHEMICAL REVIEWS, 2003, 103 (01) : 53 - 69
  • [8] TRANSIENT DOMAINS INDUCED BY INFLUENZA HEMAGGLUTININ DURING MEMBRANE-FUSION
    BLUMENTHAL, R
    PAK, CC
    RAVIV, Y
    KRUMBIEGEL, M
    BERGELSON, LD
    MORRIS, SJ
    LOWY, RJ
    [J]. MOLECULAR MEMBRANE BIOLOGY, 1995, 12 (01) : 135 - 142
  • [9] Dilation of the influenza hemagglutinin fusion pore revealed by the kinetics of individual cell-cell fusion events
    Blumenthal, R
    Sarkar, DP
    Durell, S
    Howard, DE
    Morris, SJ
    [J]. JOURNAL OF CELL BIOLOGY, 1996, 135 (01) : 63 - 71
  • [10] Fluorescent lipid probes in the study of viral membrane fusion
    Blumenthal, R
    Gallo, SA
    Viard, M
    Raviv, Y
    Puri, A
    [J]. CHEMISTRY AND PHYSICS OF LIPIDS, 2002, 116 (1-2) : 39 - 55