Effective methods for the Biotinylation of azamacrocycles

被引：27

作者：

Krivickas, Sara J.

Tamanini, Emiliano

Todd, Matthew H.

Watkinson, Michael

机构：

[1] Queen Mary Univ London, Sch Biol & Chem Sci, London E1 4NS, England

[2] Univ Sydney, Sch Chem, Sydney, NSW 2006, Australia

来源：

JOURNAL OF ORGANIC CHEMISTRY | 2007年 / 72卷 / 22期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

D O I：

10.1021/jo071175v

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The biotin-(strept)avidin interaction remains a gold standard of model biological recognition events. The biotinylation of azarnacrocycles permits the investigation of signal transduction between this recognition event and the metal center of an azamacrocycle complex, of wide potential interest in biosensing. There are no generally applicable procedures in the literature for such functionalizations. We report here a comprehensive investigation into the attachment of biotin to TACN, cyclen, and cyclam.. Effective methods have been found for each ring. The efficacy of the functionalization is critically dependent on the nature of the azarnacrocycle.

引用

收藏

页码：8280 / 8289

页数：10

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