Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum

被引：53

作者：

Gonzalez, David J. ^{[2
]}

Lee, Shaun W. ^{[9
]}

Hensler, Mary E. ^{[6
]}

Markley, Andrew L. ^{[2
]}

Dahesh, Samira ^{[6
]}

Mitchell, Douglas A. ^{[10
,11
,12
]}

Bandeira, Nuno ^{[7
]}

Nizet, Victor ^{[1
,6
]}

Dixon, Jack E. ^{[2
,4
,5
,8
]}

Dorrestein, Pieter C. ^{[1
,2
,3
,4
]}

机构：

[1] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Ctr Marine Biotechnol & Biomed, La Jolla, CA 92093 USA

[4] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA

[5] Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA

[6] Univ Calif San Diego, Div Pharmacol & Drug Discovery, Dept Pediat, La Jolla, CA 92093 USA

[7] Univ Calif San Diego, Dept Comp Sci & Engn, La Jolla, CA 92093 USA

[8] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA

[9] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA

[10] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[11] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA

[12] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2010年 / 285卷 / 36期

基金：

美国国家卫生研究院;

关键词：

STREPTOLYSIN-S; MASS-SPECTROMETRY; MICROCIN C7; BIOSYNTHESIS; PEPTIDE; STREPTOCOCCUS; IDENTIFICATION; SPOROGENES; COMPONENT; PATHWAY;

D O I：

10.1074/jbc.M110.118554

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Through elaboration of its botulinum toxins, Clostridium botulinum produces clinical syndromes of infant botulism, wound botulism, and other invasive infections. Using comparative genomic analysis, an orphan nine-gene cluster was identified in C. botulinum and the related foodborne pathogen Clostridium sporogenes that resembled the biosynthetic machinery for streptolysin S, a key virulence factor from group A Streptococcus responsible for its hallmark beta-hemolytic phenotype. Genetic complementation, in vitro reconstitution, mass spectral analysis, and plasmid intergrational mutagenesis demonstrate that the streptolysin S-like gene cluster from Clostridium sp. is responsible for the biogenesis of a novel post-translationally modified hemolytic toxin, clostridiolysin S.

引用

页码：28220 / 28228

页数：9

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