Morphological study of fully and partially isolated early human follicles

被引:115
作者
Abir, R
Fisch, B
Nitke, S
Okon, E
Raz, A
Ben Rafael, Z
机构
[1] Rabin Med Ctr, Dept Obstet & Gynecol, IVF Res Lab, IL-49100 Petah Tiqwa, Israel
[2] Tel Aviv Univ, Sackler Fac Med, Dept Obstet & Gynecol, IL-69978 Tel Aviv, Israel
[3] Tel Aviv Univ, Sackler Fac Med, Dept Pathol, IL-69978 Tel Aviv, Israel
关键词
in vitro; cryopreservation; full and partial isolation; transmission electron microscopy (TEM); primordial and primary follicles;
D O I
10.1016/S0015-0282(00)01668-X
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To compare the development of fully and partially isolated human follicles by using various culture systems. Design: Human ovarian material was incubated with collagenase and deoxyribonuclease. Fully and partially isolated follicles (30-50 mum) were dissected and studied under light and electron microscopy. The follicles were then cultured on and within various matrices. Fully isolated follicles were also cocultured with stromal cells. Setting: Rabin Medical Center, a major care and referral center. Patient(s): Women undergoing laparoscopy. Intervention(s): None. Main Outcome Measure(s): Microscopy studies, follicular measurements. Result(s): Electron microscopy studies revealed an excess of lipid droplets in the granulosa cells of freshly isolated follicles. An increase in follicular size and granulosa cell number was observed only in the fully isolated follicles cultured within collagen gels for 24 hours. Most of the partially isolated follicles detached from the collagen gels. When cultured on collagen, extracellular matrix, and poly-L-lysine, both the fully and the partially isolated follicles deteriorated within the first 24 hours; coculture with stromal cells had no beneficial effect. Conclusion(s): The excess in lipid droplets in granulosa cells of isolated follicles might suggest that the isolation process does not yield completely healthy follicles. However, despite this finding, our studies show that fully isolated follicles, but not partially isolated follicles, can grow within, but not on, a culture matrix. (C) 2001 by American Society for Reproductive Medicine.
引用
收藏
页码:141 / 146
页数:6
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