Effects of magnetic-activated cell sorting on sperm motility and cryosurvival rates

被引:56
作者
Said, TM
Grunewald, S
Paasch, U
Rasch, M
Agarwal, A
Glander, HJ
机构
[1] Cleveland Clin Fdn, Ctr Adv Res Human Reprod Infertil & Sexual Funct, Glickman Urol Inst, Cleveland, OH 44195 USA
[2] Cleveland Clin Fdn, Dept Obstet & Gynecol, Cleveland, OH 44195 USA
[3] Univ Leipzig, Dept Dermatol, Androl Unit, D-7010 Leipzig, Germany
关键词
annexin; cryosurvival rate; magnetic-activated cell sorting; sperm;
D O I
10.1016/j.fertnstert.2004.11.052
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To evaluate the effect of magnetic-activated cell sorting in cryopreservation-thawing protocols on sperm motility and cryosurvival rate. Design: Prospective-controlled study. Setting: Andrology department at a university-based medical institution. Patient(s): Ten healthy volunteer sperm donors. Intervention(s): Sperm populations were separated using annexin-V magnetic-activated cell sorting before and after the cryopreservation-thawing process. Main Outcome Measure(s): Sperm motility and cryosurvival rate. Result(s): Annexin-negative sperm separated by magnetic-activated cell sorting had statistically significantly higher motility following cryopreservation-thawing than sperm that were not separated. Similarly, annexin-negative spermatozoa also had higher cryosurvival rate than sperm cryopreserved without magnetic-activated cell sorting and sperm that were annex in-positive. Concluslon(s): Superparamagnetic annexin V-conjugated microbeads can separate spermatozoa with externalized phosphatidylserine, which is considered one of the early features of late apoptosis. The separation of a distinctive population of nonapoptotic spermatozoa with intact membranes may optimize the cryopreservation-thawing outcome. Magnetic-activated cell sorting using annexin-V microbeads enhances sperm motility and cryosurvival rates following cryopreservation. ((c) 2005 by American Society for Reproductive Medicine.)
引用
收藏
页码:1442 / 1446
页数:5
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