Influence of oocyte nuclei on demethylation of donor genome in cloned bovine embryos

被引：37

作者：

Kang, YK ^{[1
]}

Koo, DB ^{[1
]}

Park, JS ^{[1
]}

Choi, YH ^{[1
]}

Lee, KK ^{[1
]}

Han, YM ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Anim Dev Biotechnol Lab, Taejon 305600, South Korea

来源：

FEBS LETTERS | 2001年 / 499卷 / 1-2期

关键词：

epigenetic; nuclear transfer; bisulfite; reprogramming;

D O I：

10.1016/S0014-5793(01)02514-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recently demonstrated that satellite regions exhibit an aberrant DNA methylation in cloned bu,;int: embryos. Here, we examined, using bisulfite-sequencing technology, whether the inefficient demethylation of cloned donor genomes could he rescued bg the presence of oocytic nuclei. Both AciI digestion and sequencing analyses showed that satellite sequence was demethylated more efficiently in cloned tetraploid blastocysts than in diploid clones. When methyl-CpG density (the number of methyl-CpG sites per string) was scored, a significant decrease was observed in tetraploids (P < 0.001). These results suggest that unknown mechanisms provided by oocytic nuclei could assist the demethylation of satellite sequences in tetraploid clones. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：55 / 58

页数：4

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