Covalent immobilization of invertase on microporous pHEMA-GMA membrane

被引：73

作者：

Danisman, T

Tan, S ^{[1
]}

Kacar, Y

Ergene, A

机构：

[1] Kirikkale Univ, Sci & Literature Fac, Dept Biol, Kirikkale, Turkey

[2] Mersin Univ, Dept Biol, Sci & Literature Fac, Mersin, Turkey

来源：

FOOD CHEMISTRY | 2004年 / 85卷 / 03期

关键词：

pHEMA-GMA membrane; epoxy groups; covalent bonding; enzyme immobilization; invertase;

D O I：

10.1016/j.foodchem.2003.07.015

中图分类号：

O69 [应用化学];

学科分类号：

081704 [应用化学];

摘要：

Poly (2-hydroxyethyl methacrylate-glycidyl methacrylate) (pHEMA-GMA) membrane was prepared by UV-initiated photopolymerization. Invertase was immobilized by the condensation reaction of the epoxy groups of glycidyl methacrylate in the membrane structure with amino groups of the enzyme. The (Km) values were 22 mM and 58 mM for free and immobilized enzyme, respectively. Immobilization improved the pH stability and temperature stability of the enzyme. Thermal stability was found to increase with immobilization. The half times for the activity decay at 70 degreesC were found to be 11 and 38 min for the free and immobilized enzyme, respectively. The immobilized enzyme activity was found to be quite stable in later experiments. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：461 / 466

页数：6

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