Crystallization of L-aspartate oxidase, the first enzyme in the bacterial de novo biosynthesis of NAD

被引：5

作者：

Bacchella, L

Lina, C

Todone, F

Negri, A

Tedeschi, G

Ronchi, S

Mattevi, A

机构：

[1] Univ Pavia, Dipartimento Genet & Microbiol, I-27100 Pavia, Italy

[2] Univ Milan, Ist Fisiol Vet & Biochim, I-20133 Milan, Italy

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1999年 / 55卷

关键词：

D O I：

10.1107/S0907444998011913

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The flavoenzyme L-aspartate oxidase from Escherichia coli was crystallized using the hanging-drop vapour-diffusion technique with PEG 4000 as precipitant. The crystals belong to space group P3(1)21 (or P3(2)21) with unit-cell parameters a = b = 84.9, c = 159.9 Angstrom. A solvent content of 42% corresponds to a monomer (60 kDa) in the asymmetric unit. A complete 2.8 Angstrom resolution data set was collected using a rotating-anode X-ray generator.

引用

页码：549 / 551

页数：3

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