Long noncoding RNA MIR31HG is activated by SP1 and promotes cell migration and invasion by sponging miR-214 in NSCLC

Long non-coding RNAs(lncRNAs) have been reported to play pivotal roles in various cancers. Recently, MIR31HG was proposed to be involved in tumor progression. However, its role in non small cell lung cancer(NSCLC) remains elusive. In this work, we found that SP1-induced MIR31HG was significantly upregulated in NSCLC tissues and cell lines. Moreover, Cox multivariate survival analysis revealed that high MIR31HG was an independent predictor of poor overall survival(OS). Functionally, knockdown of TINCR obviously suppressed NSCLC cells migration and invasion in vitro and inhibited NSCLC cells metastasis in vivo. Mechanistically, we identified MIR31HG could act as a miR-214 sponge using RNA pull down, luciferase reporter and RIP assays. Lastly, we verified that over expression of MIR31HG effectively reverses miR-214-induced inhibition of NSCLC cells progression. Therefore, MIR31HG might serve as a promising prognostic marker and potential therapeutic target for NSCLC patients.