In vitro scratch assay:: a convenient and inexpensive method for analysis of cell migration in vitro

被引：3603

作者：

Liang, Chun-Chi ^{[1
]}

Park, Ann Y. ^{[1
]}

Guan, Jun-Lin ^{[1
]}

机构：

[1] Univ Michigan, Sch Med, Div Mol Med & Genet & Cell & Dev Biol, Dept Internal Med, Ann Arbor, MI 48109 USA

来源：

NATURE PROTOCOLS | 2007年 / 2卷 / 02期

关键词：

D O I：

10.1038/nprot.2007.30

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The in vitro scratch assay is an easy, low-cost and well-developed method to measure cell migration in vitro. The basic steps involve creating a "scratch'' in a cell monolayer, capturing the images at the beginning and at regular intervals during cell migration to close the scratch, and comparing the images to quantify the migration rate of the cells. Compared to other methods, the in vitro scratch assay is particularly suitable for studies on the effects of cell - matrix and cell - cell interactions on cell migration, mimic cell migration during wound healing in vivo and are compatible with imaging of live cells during migration to monitor intracellular events if desired. Besides monitoring migration of homogenous cell populations, this method has also been adopted to measure migration of individual cells in the leading edge of the scratch. Not taking into account the time for transfection of cells, in vitro scratch assay per se usually takes from several hours to overnight.

引用

页码：329 / 333

页数：5

共 5 条

[1] Regulation of focal adhesion kinase by a novel protein inhibitor FIP200 [J].

Abbi, S ;

Ueda, H ;

Zheng, CH ;

Cooper, LA ;

Zhao, JH ;

Christopher, R ;

Guan, JL .

MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (09) :3178-3191

[2] Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCζ [J].

Etienne-Manneville, S ;

Hall, A .

CELL, 2001, 106 (04) :489-498

[3] Phosphorylation of adducin by rho-kinase plays a crucial role in cell motility [J].

Fukata, Y ;

Oshiro, N ;

Kinoshita, N ;

Kawano, Y ;

Matsuoka, Y ;

Bennett, V ;

Matsuura, Y ;

Kaibuchi, K .

JOURNAL OF CELL BIOLOGY, 1999, 145 (02) :347-361

[4]

HAUDENSCHILD CC, 1979, LAB INVEST, V41, P407

[5] INITIATION OF CELL DIVISION IN A CONTACT-INHIBITED MAMMALIAN CELL LINE [J].

TODARO, GJ ;

LAZAR, GK ;

GREEN, H .

JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY, 1965, 66 (3P1) :325-+

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