Identification and characterization of a Sac domain-containing phosphoinositide 5-phosphatase

被引：42

作者：

Minagawa, T ^{[1
]}

Ijuin, T ^{[1
]}

Mochizuki, Y ^{[1
]}

Takenawa, T ^{[1
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Dept Biochem, Minato Ku, Tokyo 1088639, Japan

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2001年 / 276卷 / 25期

关键词：

D O I：

10.1074/jbc.M101579200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have characterized a novel Sac domain-containing inositol phosphatase, hSac2. It was ubiquitously expressed but especially abundant in the brain, heart, skeletal muscle, and kidney. Unlike other Sac domain containing proteins, hSac2 protein exhibited B-phosphatase activity specific for phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. This is the first time that the Sac domain has been reported to possess 5-phosphatase activity. Its B-phosphatase activity for phosphatidylinositol 4,5-bisphosphate (K-m = 14.3 muM) was comparable with those of Type II 5-phosphatases. These results imply that hSac2 functions as an inositol polyphosphate 5-phosphatase.

引用

页码：22011 / 22015

页数：5

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