Oral delivery and gastrointestinal absorption of soluble glucans stimulate increased resistance to infectious challenge

被引:246
作者
Rice, PJ
Adams, EL
Ozment-Skelton, T
Gonzalez, AJ
Goldman, MP
Lockhart, BE
Barker, LA
Breuel, KF
DePonti, WK
Kalbfleisch, JH
Ensley, HE
Brown, GD
Gordon, S
Williams, DL
机构
[1] E Tennessee State Univ, Coll Med, Dept Pharmacol, Johnson City, TN 37614 USA
[2] E Tennessee State Univ, Coll Med, Dept Surg, Johnson City, TN 37614 USA
[3] E Tennessee State Univ, Coll Med, Dept Obstet & Gynecol, Johnson City, TN 37614 USA
[4] E Tennessee State Univ, Coll Med, Dept Biometry & Med Comp, Johnson City, TN 37614 USA
[5] Tulane Univ, Dept Chem, New Orleans, LA 70118 USA
[6] Univ Oxford, Sir William Dunn Sch Pathol, Oxford, England
关键词
D O I
10.1124/jpet.105.085415
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Glucans are immunomodulatory carbohydrates found in the cell walls of fungi and certain bacteria. We examined the pharmacokinetics of three water-soluble glucans ( glucan phosphate, laminarin, and scleroglucan) after oral administration of 1 mg/kg doses in rats. Maximum plasma concentrations for glucan phosphate occurred at 4 h. In contrast, laminarin and scleroglucan showed two plasma peaks between 0.5 and 12 h. At 24 h, 27 +/- 3% of the glucan phosphate and 20 +/- 7% of the laminarin remained in the serum. Scleroglucan was rapidly absorbed and eliminated. The liver did not significantly contribute to the clearance of plasma glucan. Biological effects were further studied in mice. Following oral administration of 1 mg, glucans were bound and internalized by intestinal epithelial cells and gut-associated lymphoid tissue (GALT) cells. Internalization of glucan by intestinal epithelial cells was not Dectin-dependent. GALT expression of Dectin-1 and toll-like receptor (TLR)2, but not TLR4, increased following oral administration of glucan. Oral glucan increased systemic levels of interleukin (IL)-12 (151 +/- 15%) in mice. Oral glucan administration also increased survival in mice challenged with Staphylococcus aureus or Candida albicans. These data demonstrate that orally administered water-soluble glucans translocate from the gastrointestinal (GI) tract into the systemic circulation. The glucans are bound by GI epithelial and GALT cells, and they modulate the expression of pattern recognition receptors in the GALT, increase IL-12 expression, and induce protection against infectious challenge.
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收藏
页码:1079 / 1086
页数:8
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