Comparison of rectoanal mucosal swab cultures and fecal cultures for determining prevalence of Escherichia coli O157:H7 in feedlot cattle

被引：50

作者：

Greenquist, MA

Drouillard, JS

Sargeant, JM

Depenbusch, BE

Shi, XR

Lechtenberg, KF

Nagaraja, TG

机构：

[1] Kansas State Univ, Dept Anim Sci & Ind, Manhattan, KS 66505 USA

[2] Kansas State Univ, Dept Diagnost Med & Pathobiol, Manhattan, KS 66505 USA

[3] Midwest Vet Serv Inc, Oakland, NE 68045 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 10期

关键词：

D O I：

10.1128/AEM.71.10.6431-6433.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We compared fecal samples with samples collected with rectoanal mucosa swabs (RAMS) to determine the prevalence of Escherichia coli O157 in feedlot cattle (n = 747). Escherichia coli O157 was detected in 9.5% of samples collected with RAMS and 4.7% of samples tested by fecal culture. Pulsed-field gel electrophoresis analysis of isolates suggested that the strains colonizing the rectoanal junction were the same as those from the feces. Mucosal swab sampling was more sensitive than fecal sampling for determining the prevalence of E. coli O157 in feedlot cattle.

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页码：6431 / 6433

页数：3

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