ACE2 receptor expression and severe acute respiratory syndrome coronavirus infection depend on differentiation of human airway epithelia

被引:604
作者
Jia, HP
Look, DC
Shi, L
Hickey, M
Pewe, L
Netland, J
Farzan, M
Wohlford-Lenane, C
Perlman, S
McCray, PB
机构
[1] Univ Iowa, Carver Coll Med, Dept Pediat, Iowa City, IA 52242 USA
[2] Univ Iowa, Carver Coll Med, Dept Internal Med, Iowa City, IA 52242 USA
[3] Harvard Univ, Dept Microbiol & Mol Genet, Cambridge, MA 02138 USA
关键词
D O I
10.1128/JVI.79.23.14614-14621.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Studies of patients with severe acute respiratory syndrome (SARS) demonstrate that the respiratory tract is a major site of SARS-coronavirus (CoV) infection and disease morbidity. We studied host-pathogen interactions using native lung tissue and a model of well-differentiated cultures of primary human airway epithelia. Angiotensin converting enzyme 2 (ACE2), the receptor for both the SARS-CoV and the related human respiratory coronavirus NL63, was expressed in human airway epithelia as well as lung parenchyma. As assessed by immunofluorescence staining and membrane biotinylation, ACE2 protein was more abundantly expressed on the apical than the basolateral surface of polarized airway epithelia. Interestingly, ACE2 expression positively correlated with the differentiation state of epithelia. Undifferentiated cells expressing little ACE2 were poorly infected with SARS-CoV, while well-differentiated cells expressing more ACE2 were readily infected. Expression of ACE2 in poorly differentiated epithelia facilitated SARS spike (S) protein-pseudotyped virus entry. Consistent with the expression pattern of ACE2, the entry of SARS-CoV or a lentivirus pseudotyped with SARS-CoV S protein in differentiated epithelia was more efficient when applied to the apical surface. Furthermore, SARS-CoV replicated in polarized epithelia and preferentially exited via the apical surface. The results indicate that infection of human airway epithelia by SARS coronavirus correlates with the state of cell differentiation and ACE2 expression and localization. These findings have implications for understanding disease pathogenesis associated with SARS-CoV and NL63 infections.
引用
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页码:14614 / 14621
页数:8
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