Polyphenol-enriched fractions from Sicilian grape pomace: HPLC-DAD analysis and antioxidant activity

被引：66

作者：

Amico, V. ^{[1
]}

Chillemi, R. ^{[1
]}

Mangiafico, S. ^{[1
]}

Spatafora, C. ^{[1
]}

Tringali, C. ^{[1
]}

机构：

[1] Univ Catania, Dipartimento Sci Chim, I-95125 Catania, Italy

来源：

BIORESOURCE TECHNOLOGY | 2008年 / 99卷 / 13期

关键词：

antioxidant polyphenols; grape pomace; HPLC-DAD; DPPH center dot; GAE;

D O I：

10.1016/j.biortech.2007.10.037

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

On the basis of a preliminary screening of seven different samples of Sicilian grape pomace, the 'Nerello Mascalese' sample NM2 was selected for an ethanol preparative extraction. The defatted NM2 EtOH extract was subjected to DPPH center dot and GAE assays, showing good radical scavenging activity (SC50 = 9.9 mu g/mL) and a GAE value of 397.7 mg/g extract. HPLC-DAD analysis of NM2 extract allowed a quantitative determination of the main anthocyanins (AN) and flavonols/flavonol glycosides (FL/FG). Aliquots of the NM2 extract were subjected to three different fractionation protocols (FP1, FP2 and FP3). The fractions were examined by DPPH center dot and GAE assays, and subjected to HPLC-DAD analysis for the quantitative determination of the main AN and FL/FG. FP3 allowed obtaining a polyphenol-enriched fraction with SC50 = 14.8 mu g/mL and GAE = 184.1 mg/g of fraction, accounting for only 1.3% in weight of the EtOH extract. Some considerations about the relationship between antioxidant activity and AN/FL/FG HPLC-DAD profiles are also reported. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：5960 / 5966

页数：7

共 30 条

[1] Antidiabetic activity of red wine polyphenolic extract, ethanol, or both in streptozotocin-treated rats [J].

Al-Awwadi, N ;

Azay, J ;

Poucheret, P ;

Cassanas, G ;

Krosniak, M ;

Auger, C ;

Gasc, F ;

Rouanet, JM ;

Cros, G ;

Teissèdre, PL .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (04) :1008-1016

[2] Antiproliferative terpenoids from almond hulls (Prunus dulcis):: Identification and structure-activity relationships [J].

Amico, V ;

Barresi, V ;

Condorelli, D ;

Spatafora, C ;

Tringali, C .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (03) :810-814

[3] Constituents of grape pomace from the Sicilian cultivar 'Nerello Mascalese' [J].

Amico, V ;

Napoli, EM ;

Renda, A ;

Ruberto, G ;

Spatafora, C ;

Tringali, C .

FOOD CHEMISTRY, 2004, 88 (04) :599-607

[4]

[Anonymous], 2006, FAO STAT DATABASE

[5] Wine flavonoids protect against LDL oxidation and atherosclerosis [J].

Aviram, M ;

Fuhrman, B .

ALCOHOL AND WINE IN HEALTH AND DISEASE, 2002, 957 :146-161

[6]

BRAND-WILLIAMS W, 1995, FOOD SCI TECHNOL-LEB, V28, P25

[7]

Damianaki A, 2000, J CELL BIOCHEM, V78, P429, DOI 10.1002/1097-4644(20000901)78:3<429::AID-JCB8>3.0.CO

[8]

2-M

[9] Naphthalene diols: A new class of antioxidants intramolecular hydrogen bonding in catechols, naphthalene diols, and their aryloxyl radicals [J].

Foti, MC ;

Johnson, ER ;

Vinqvist, MR ;

Wright, JS ;

Barclay, LRC ;

Ingold, KU .

JOURNAL OF ORGANIC CHEMISTRY, 2002, 67 (15) :5190-5196

[10]

GHEDIRA K, 2005, PHYTOTHERAPIE, V4, P162

← 1 2 3 →