Polypyrrole-coated glucose oxidase nanoparticles for biosensor design

被引：116

作者：

Ramanavicius, A ^{[1
]}

Kausaitè, A

Ramanavicienè, A

机构：

[1] Vilnius State Univ, Dept Analyt & Environm Chem, LT-03225 Vilnius, Lithuania

[2] Vilnius State Univ, Inst Immunol, Sector Immunoanal & Informat, LT-08409 Vilnius, Lithuania

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2005年 / 111卷

关键词：

conducting polymers; glucose oxidase; biosensor; nanoparticles; polypyrrole; biocatalysis; nanotechnology;

D O I：

10.1016/j.snb.2005.03.038

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Self-encapsulation of redox enzyme-glucose oxidase E.C. 1.1.3.4. from Penecillum vitale (GOX)-within conducting polymer polypyrrole (Ppy) is firstly described. Polymerization of polypyrrole was initiated by GOX catalytic action product-hydrogen peroxide. It was acting as oxidator generating free radicals and initiating the polymerization of pyrrole. The increase in optical absorbance at 460 nm was exploited for the monitoring of polypyrrole polymerization process. The presence of entrapped glucose oxidase within polypyrrole was determined by basic application of polypyrrole-coated glucose oxidase nanoparticles (GOX/Ppy) in electrochemical biosensor design. The increase of K-M by over 10 times was determined for polypyrrole-coated GOX, if compared with native GOX. Further application of nanoparticles based on self-encapsulated glucose oxidase and other oxidases is predicted for various bioanalytical purposes and other biocatalytic applications. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：532 / 539

页数：8

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