Molecular cloning and expression profiles of Argonaute proteins in Schistosoma japonicum

被引：21

作者：

Chen, Jing ^{[1
,2
]}

Yang, Yanping ^{[1
,3
]}

Guo, Suxia ^{[1
]}

Peng, Jinbiao ^{[1
]}

Liu, Zongping ^{[3
]}

Li, Jiakui ^{[2
]}

Lin, Jiaojiao ^{[1
]}

Cheng, Guofeng ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Lab Anim Parasitol, Minist Agr, Shanghai 200241, Peoples R China

[2] Huazhong Agr Univ, Coll Vet Med, Wuhan, Peoples R China

[3] Yangzhou Univ, Coll Vet Med, Yangzhou, Peoples R China

来源：

PARASITOLOGY RESEARCH | 2010年 / 107卷 / 04期

基金：

中国国家自然科学基金;

关键词：

IN-VITRO; RNA; IDENTIFICATION; INTERFERENCE; TRANSLATION; MICRORNAS; FAMILY;

D O I：

10.1007/s00436-010-1946-3

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程]; 100103 [病原生物学];

摘要：

Small non-coding RNAs including microRNAs and small interfering RNAs play important roles in many biological processes of many organisms. Argonaute proteins serve as a key component of the RNA-induced silencing complex for mediating miRNA/siRNA functions. In the present study, we systematically investigated Argonaute proteins in Schistosoma japonicum by using bioinformatics in combination with 5'- and 3'-Rapid Amplification of cDNA ends techniques and thus obtained three full-length cDNAs encoding Argonaute proteins, named as SjAgo1, SjAgo2, and SjAgo3, respectively. Additionally, SjAgo1/2/3 were differentially expressed in different developmental stages of schistosomes as determined by real-time RT-PCR and Western blot. Taken together, our preliminary results suggested that SjAgo1/2/3 may control gene expression during the life cycle of S. japonicum and therefore may regulate schistosome development and other biological processes.

引用

页码：889 / 899

页数：11

共 28 条

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