Production of knockout rats using ENU mutagenesis and a yeast-based screening assay

被引：150

作者：

Zan, YH ^{[1
]}

Haag, JD ^{[1
]}

Chen, KS ^{[1
]}

Shepel, LA ^{[1
]}

Wigington, D ^{[1
]}

Wang, YR ^{[1
]}

Hu, R ^{[1
]}

Lopez-Guajardo, CC ^{[1
]}

Brose, HL ^{[1
]}

Porter, KI ^{[1
]}

Leonard, RA ^{[1
]}

Hitt, AA ^{[1
]}

Schommer, SL ^{[1
]}

Elegbede, AF ^{[1
]}

Gould, MN ^{[1
]}

机构：

[1] Univ Wisconsin, McArdle Lab Canc Res, Dept Oncol, Madison, WI 53706 USA

来源：

NATURE BIOTECHNOLOGY | 2003年 / 21卷 / 06期

关键词：

D O I：

10.1038/nbt830

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The rat is a widely used model in biomedical research and is often the preferred rodent model in many areas of physiological and pathobiological research. Although many genetic tools are available for the rat, methods to produce gene-disrupted knockout rats are greatly needed. In this study, we developed protocols for creating N-ethyl-N-nitrosourea (ENU)-induced germline mutations in several rat strains. F-1 preweanling pups from mutagenized Sprague Dawley (SD) male rats were then screened for functional mutations in Brca1 and Brca2 using a yeast gap-repair, ADE2-reporter truncation assay. We produced knockout rats for each of these two breast cancer suppressor genes.

引用

页码：645 / 651

页数：7

共 21 条

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