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A low-cost, disposable card for rapid polymerase chain reaction

被引：22
作者：
Jia, Guangyao
Siegrist, Jonathan
Deng, Chengwu
Zoval, Jim V.
Stewart, Gale
Peytavi, Regis
Huletsky, Ann
Bergeron, Michel G.
Madou, Marc J. [1 ]
机构：
[1] Univ Calif Irvine, Dept Mech & Aerosp Engn, Irvine, CA 92697 USA
[2] Univ Quebec, Ctr Hosp, Ctr Rech & Infectiol, Ste Foy, PQ, Canada
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2007年 / 58卷 / 01期
关键词：
PCR amplification; disposable card; ice valve; miniaturization; modeling;
D O I：
10.1016/j.colsurfb.2007.03.007
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A low-cost, disposable card for rapid polymerase chain reaction (PCR) was developed in this work. Commercially available, adhesive-coated aluminum foils and polypropylene films were laminated with structured polycarbonate films to form microreactors in a card format. Ice valves [I] were employed to seat the reaction chambers during thermal cycling and a Peltier-based thermal cycler was configured for rapid thermal cycling and ice valve actuation. Numerical modeling was conducted to optimize the design of the PCR reactor and investigate the thermal gradient in the reaction chamber in the direction of sample thickness. The PCR reactor was experimentally characterized by using thin foil thermocouples and validated by a successful amplification of 10 copy of E. coli tuf gene in 27 min. (c) 2007 Elsevier B.V. All rights reserved.
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收藏
页码：52 / 60
页数：9
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