Development of an immunoassay for monitoring the levels of ciprofloxacin in patient samples

被引:26
作者
Snitkoff, GG
Grabe, DW
Holt, R
Bailie, GR
机构
[1] Albany Coll Pharm, Dept Sci Biol, Albany, NY 12208 USA
[2] Albany Coll Pharm, Dept Pharm Practice, Albany, NY 12208 USA
来源
JOURNAL OF IMMUNOASSAY | 1998年 / 19卷 / 04期
关键词
immunoassay; antibiotic; ciprofloxacin;
D O I
10.1080/01971529808005483
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It has been traditional to measure drug concentrations-using high pressure liquid chromatography (HPLC). While this method is highly accurate, it is time consuming and requires the use of appropriate standards for identification of the compound. In addition, identification and quantification of drugs from patient samples requires significant manipulation to remove protein. In contrast, enzyme-linked immunoassays (EIA) are able to assay samples with a high degree of specificity, and are able to process. multiple samples at a time. In addition, serum proteins do not interfere with sample quantification and samples may be tested without significant preparation. We describe the development of an EIA for the detection of ciprofloxacin in serum and dialysate samples. The immunoassay is specific for ciprofloxacin and is sensitive for picogram amounts of the antibiotic.
引用
收藏
页码:227 / 238
页数:12
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