Biorecognition - Gated ion channel biosensors - Membranes supported by poly-1,2-diaminobenzene

被引：1

作者：

Anrather, D ^{[1
]}

Valina-Saba, M ^{[1
]}

Smetazko, M ^{[1
]}

Bauer, G ^{[1
]}

Schmutzer, S ^{[1
]}

Weiss-Wichert, C ^{[1
]}

Schalkhammer, T ^{[1
]}

机构：

[1] Univ Vienna, Inst Biochem & Mol Zellbiol, Wien, Austria

来源：

BIOMEDICAL SENSING AND IMAGING TECHNOLOGIES, PROCEEDINGS OF | 1998年 / 3253卷

关键词：

supported membranes; 1,2-diaminobenzene; Bisgramicidin-A; ion channels;

D O I：

10.1117/12.308017

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The device presented is based on a supported membrane incorporating modified ion channels. Various analytes induced down regulation of the ion flux by interaction with specific binding sites (metal ions) or artificial ligands (antibodies) attached near the channel entrance. To set up such a device new types of stabilizing membrane supports had to be developed. The new support presented was deposited by electropolymerization of 1,2-diaminobenzene onto metal electrodes thus exhibiting a highly charged surface. Negatively charged lipids formed SA-membranes tightly bound to that gel surface. Mixtures of biological lipids and archaeabacteria type bolaamphiphilic lipids minimized floating of the membrane layer. Various ligands were bound to the modified Bisgramicidin-A to interact with their specific antibodies. It turned out that it is vital to accurately couple the ligand at a single functional hydroxy-group. Sensors were optimized using a metal/lgand and 2,4-dinitrophenol/polyclonal antibody setup. Summing up, highly stable supported membranes were formed, ion channel were functionally integrated into these membranes and molecule interactions of analytes with embedded ion channels were monitored.

引用

页码：2 / 11

页数：10