2,4-dinitrobenzenesulfonyl fluoresceins as fluorescent alternatives to Ellman's reagent in thiol-quantification enzyme assays

被引：354

作者：

Maeda, H ^{[1
]}

Matsuno, H ^{[1
]}

Ushida, M ^{[1
]}

Katayama, K ^{[1
]}

Saeki, K ^{[1
]}

Itoh, N ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Pharmaceut Sci, Div Analyt Chem, Suita, Osaka 5650871, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 19期

关键词：

enzymes; fluorescence; high-throughput screening inhibitors; sensors;

D O I：

10.1002/anie.200500114

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Fluorescent sensor for thiols: Deprotection of nonfluorescent 1 by thiols (R′SH) proceeds rapidly and near-quantitatively in aqueous solution (pH 7.4) to produce highly fluorescent 2. Assays performed in the presence of 1 provide a rapid and simple method for the determination of inhibitory constants for inhibitors such as donepezil toward acetyl- and butyrylcholinesterases. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2922 / 2925

页数：4

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