Genetic analysis of microcin H47 immunity

被引：21

作者：

Rodriguez, E ^{[1
]}

Lavina, M ^{[1
]}

机构：

[1] Fac Ciencias, Secc Fisiol & Genet Bacterianas, Montevideo 11200, Uruguay

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1998年 / 44卷 / 07期

关键词：

microcin H47; microcin immunity; phoA gene fusion; membrane peptide;

D O I：

10.1139/cjm-44-7-692

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Microcin H47 is a bactericidal antibiotic produced by a natural Escherichia coli isolate. The genetic system encoding microcin production and immunity consists of at least seven clustered genes. Four of these are devoted to microcin biosynthesis and two genes are required for its secretion into the extracellular medium. The product of the seventh gene, mchI, determines the cell's self-immunity. This gene was shown to encode a highly hydrophobic 69-residue peptide. Analysis of the MchI amino acid sequencer as well as the characterization of MchI-PhoA hybrid proteins. indicated that the microcin immunity product is probably exported out of the cytoplasm and remains an integral membrane peptide. This localization of the immunity peptide points to the cellular membrane as the site of action of microcin H47.

引用

页码：692 / 697

页数：6

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