Primer specific and mispair extension analysis (PSMEA) as a simple approach to fast genotyping

被引：20

作者：

Hu, YW

Balaskas, E

Kessler, G

Issid, C

Scully, LJ

Murphy, DG

Rinfret, A

Giulivi, A

Scalia, V

Gill, P

机构：

[1] Canadian Red Cross Soc, Ottawa, ON K1G 4J5, Canada

[2] Lab Sante Publ Quebec, St Anne De Bellevue, PQ H9X 3R5, Canada

[3] CHUM, Res Ctr, Montreal, PQ H2X 1P1, Canada

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 21期

关键词：

D O I：

10.1093/nar/26.21.5013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method, primer specific and mispair extension analysis (PSMEA) with pfu DNA polymerase was developed for genotyping, PSMEA is based on the unique properties of 3'-->5' exonuclease proofreading activity, In the presence of an incomplete set of dNTPs, pfu was found to be extremely discriminative in nucleotide incorporation and proofreading at the initiation step of DNA synthesis, completely preventing primer extension when mispair(s) are found adjacent to the 3'-end of the primer. This has allowed us to accurately detect nucleotide variations, deletions and insertions for fast genotyping.

引用

页码：5013 / 5015

页数：3

共 6 条

[1] HIGH-FIDELITY AMPLIFICATION USING A THERMOSTABLE DNA-POLYMERASE ISOLATED FROM PYROCOCCUS-FURIOSUS [J].