Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods

被引：188

作者：

Gratton, E ^{[1
]}

Breusegem, S ^{[1
]}

Sutin, J ^{[1
]}

Ruan, QQ ^{[1
]}

机构：

[1] Univ Illinois, Fluorescence Dynam Lab, Urbana, IL 61801 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2003年 / 8卷 / 03期

关键词：

fluorescence lifetime imaging; frequency domain; time domain; two-photon microscopy;

D O I：

10.1117/1.1586704

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence lifetime images are obtained with the laser scanning microscope using two methods: the time-correlated single-photon counting method and the frequency-domain method. In the same microscope system, we implement both methods. We perform a comparison of the performance of the two approaches in terms of signal-to-noise ratio (SNR) and the speed of data acquisition. While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples. (C) 2003 Society of Photo-Optical.

引用

页码：381 / 390

页数：10

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