Photosynthesis is strongly reduced by antisense suppression of chloroplastic cytochrome bf complex in transgenic tobacco

被引:77
作者
Price, GD
von Caemmerer, S
Evans, JR
Siebke, K
Anderson, JM
Badger, MR
机构
[1] Australian Natl Univ, Res Sch Biol Sci, Mol Plant Physiol Grp, Canberra, ACT 2601, Australia
[2] Australian Natl Univ, Environm Biol Grp, Canberra, ACT 2601, Australia
[3] Australian Natl Univ, Res Sch Biol Sci, PhotoBioenerget Grp, Canberra, ACT 2601, Australia
[4] CSIRO, Div Plant Ind, Canberra, ACT 2601, Australia
来源
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY | 1998年 / 25卷 / 04期
关键词
antisense RNA; chloroplastic cytochrome bf complex; metabolite pools; Nicotiana (transgenic plants); photosynthesis; electron transport; Rieske; stomata;
D O I
10.1071/PP97164
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We have used transgenic tobacco (Nicotiana tabacum L. cv. W38) plants expressing an antisense gene directed against the transcript of the Rieske FeS protein of the chloroplast bf complex to examine the effect a reduction in chloroplast Rieske FeS content on leaf gas exchange and photosynthetic metabolite pools. Plants with chloroplast Rieske FeS protein content ranging from 5 to 80% of wild-type were analysed. CO2 assimilation rate declined linearly with the reduction in Rieske FeS content when expressed on a leaf area basis. In contrast to photosynthesis, there was no change in stomatal conductance except for plants with less than 10% of wild-type Rieske FeS content. There was a close correlation between Rieske FeS content and cytochrome f content, showing that the reduction in Rieske FeS content lead to a similar reduction in other components of the cytochrome bf complex. While lower Rieske FeS content was associated with declines in chlorophyll content, ATP delta subunit and ribulosebisphosphate carboxylase-oxygenase (Rubisco) contents, these declines were considerably smaller than the reduction in cytochrome bf content. As Rieske FeS content was reduced, there was a slight lowering of the chlorophyll a/b ratio. Lower CO2 assimilation rates at ambient CO2 and high light were associated with dramatic reductions in ribulose bisphosphate (RuBP) and modest declines in 3-phosphoglycerate (PGA). Rubisco carbamylation declined to 40-50% in plants with Rieske FeS content lower than 20% of wild-type. We conclude that, at high light, a reduction in chloroplast Rieske FeS protein content leads to inhibition of CO2 assimilation rate via reductions in the rate of RuBP regeneration caused by a restriction in electron transport and via effects on in vivo Rubisco activity.
引用
收藏
页码:445 / 452
页数:8
相关论文
共 39 条
[1]   REDOX CONTROL OF GENE-EXPRESSION AND THE FUNCTION OF CHLOROPLAST GENOMES - AN HYPOTHESIS [J].
ALLEN, JF .
PHOTOSYNTHESIS RESEARCH, 1993, 36 (02) :95-102
[3]   CYTOCHROME-B6F COMPLEX - DYNAMIC MOLECULAR-ORGANIZATION, FUNCTION AND ACCLIMATION [J].
ANDERSON, JM .
PHOTOSYNTHESIS RESEARCH, 1992, 34 (03) :341-357
[4]   PHOTOREGULATION OF THE COMPOSITION, FUNCTION, AND STRUCTURE OF THYLAKOID MEMBRANES [J].
ANDERSON, JM .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1986, 37 :93-136
[5]   Reduced levels of cytochrome bf complex in transgenic tobacco leads to marked photochemical reduction of the plastoquinone pool, without significant change in acclimation to irradiance [J].
Anderson, JM ;
Price, GD ;
Chow, WS ;
Hope, AB ;
Badger, MR .
PHOTOSYNTHESIS RESEARCH, 1997, 53 (2-3) :215-227
[6]   THE RELATIONSHIP BETWEEN STEADY-STATE GAS-EXCHANGE OF BEAN-LEAVES AND THE LEVELS OF CARBON-REDUCTION-CYCLE INTERMEDIATES [J].
BADGER, MR ;
SHARKEY, TD ;
VONCAEMMERER, S .
PLANTA, 1984, 160 (04) :305-313
[7]   CHLOROPLAST GENE-EXPRESSION IN NUCLEAR, PHOTOSYNTHETIC MUTANTS OF MAIZE [J].
BARKAN, A ;
MILES, D ;
TAYLOR, WC .
EMBO JOURNAL, 1986, 5 (07) :1421-1427
[8]   ACTIVITY RATIOS OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE ACCURATELY REFLECT CARBAMYLATION RATIOS [J].
BUTZ, ND ;
SHARKEY, TD .
PLANT PHYSIOLOGY, 1989, 89 (03) :735-739
[9]  
CRAMER WA, 1991, CURR TOP BIOENERG, V16, P179
[10]   LIGHT-INTENSITY REGULATION OF CAB GENE-TRANSCRIPTION IS SIGNALED BY THE REDOX STATE OF THE PLASTOQUINONE POOL [J].
ESCOUBAS, JM ;
LOMAS, M ;
LAROCHE, J ;
FALKOWSKI, PG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (22) :10237-10241