A functionally characterized test set of human induced pluripotent stem cells

被引：359

作者：

Boulting, Gabriella L. ^{[1
,2
,3
]}

Kiskinis, Evangelos ^{[1
,2
]}

Croft, Gist F. ^{[4
,5
,6
,7
]}

Amoroso, Mackenzie W. ^{[4
,5
,6
,7
]}

Oakley, Derek H. ^{[4
,5
,6
,7
]}

Wainger, Brian J. ^{[8
,9
,10
,11
]}

Williams, Damian J. ^{[12
,13
]}

Kahler, David J. ^{[14
]}

Yamaki, Mariko ^{[1
,2
]}

Davidow, Lance ^{[2
]}

Rodolfa, Christopher T. ^{[3
]}

Dimos, John T. ^{[3
]}

Mikkilineni, Shravani ^{[2
,3
]}

MacDermott, Amy B. ^{[12
,13
]}

Woolf, Clifford J. ^{[8
,9
,10
]}

Henderson, Christopher E. ^{[4
,5
,6
,7
]}

Wichterle, Hynek ^{[4
,5
,6
,7
]}

Eggan, Kevin ^{[1
,2
,3
]}

机构：

[1] Howard Hughes Med Inst, Cambridge, MA USA

[2] Harvard Univ, Harvard Stem Cell Inst, Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA

[3] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[4] Columbia Univ, Jenifer Estess Lab Stem Cell Res, Project ALS, New York, NY USA

[5] Columbia Univ, Ctr Motor Neuron Biol & Dis MNC, Dept Pathol, New York, NY USA

[6] Columbia Univ, Ctr Motor Neuron Biol & Dis MNC, Dept Neurol & Neurosci, New York, NY USA

[7] Columbia Stem Cell Initiat CSCI, New York, NY USA

[8] Childrens Hosp, Program Neurobiol, Boston, MA 02115 USA

[9] Childrens Hosp, FM Kirby Neurobiol Ctr, Boston, MA 02115 USA

[10] Harvard Univ, Sch Med, Dept Neurobiol, Boston, MA 02115 USA

[11] Massachusetts Gen Hosp, Dept Anesthesia Crit Care & Pain Med, Boston, MA 02114 USA

[12] Columbia Univ, Dept Physiol & Cellular Biophys, New York, NY USA

[13] Columbia Univ, Dept Neurosci, New York, NY USA

[14] New York Stem Cell Fdn Inc, New York, NY USA

来源：

NATURE BIOTECHNOLOGY | 2011年 / 29卷 / 03期

基金：

美国国家卫生研究院;

关键词：

DIRECTED DIFFERENTIATION; NEURAL CONVERSION; HUMAN ES; TISSUE; LINES; ALS;

D O I：

10.1038/nbt.1783

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Human induced pluripotent stem cells (iPSCs) present exciting opportunities for studying development and for in vitro disease modeling. However, reported variability in the behavior of iPSCs has called their utility into question. We established a test set of 16 iPSC lines from seven individuals of varying age, sex and health status, and extensively characterized the lines with respect to pluripotency and the ability to terminally differentiate. Under standardized procedures in two independent laboratories, 13 of the iPSC lines gave rise to functional motor neurons with a range of efficiencies similar to that of human embryonic stem cells (ESCs). Although three iPSC lines were resistant to neural differentiation, early neuralization rescued their performance. Therefore, all 16 iPSC lines passed a stringent test of differentiation capacity despite variations in karyotype and in the expression of early pluripotency markers and transgenes. This iPSC and ESC test set is a robust resource for those interested in the basic biology of stem cells and their applications.

引用

页码：279 / U147

页数：10

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