Amplified fluorescent molecular probes based on 1,3,5,7-tetrasubstituted adamantane

A novel series of fluorescent labels containing multiple fluorescein moieties attached to a bridgehead-substituted adamantane has been synthesized. Molecules containing two, three or four fluorescein residues were prepared and their fluorescence was measured. The rigid adamantane core prevents fluorescein moieties from intramolecular collisions, thus precluding mutual fluorescence quenching. proposed design allows building the molecular probes with amplified fluorescence signal by combining several fluorophores in a molecule. (C) 1998 Elsevier Science Ltd. All rights reserved.