Probing ion channel conformational dynamics using simultaneous single-molecule ultrafast spectroscopy and patch-clamp electric recording

被引：20

作者：

Harms, G ^{[1
]}

Orr, G ^{[1
]}

Lu, HP ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Fundamental Sci Div, Richland, WA 99352 USA

来源：

APPLIED PHYSICS LETTERS | 2004年 / 84卷 / 10期

关键词：

D O I：

10.1063/1.1652228

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

An approach to probing single-molecule ion channel kinetics and conformational dynamics, patch-clamp confocal fluorescence microscopy (PCCFM), uses simultaneous ultrafast fluorescence spectroscopy and single-channel electric current recording. PCCFM is applied to determine single-channel conformational dynamics by probing single-pair fluorescence resonant energy transfer, fluorescence self-quenching, and anisotropy of the dye-labeled gramicidin ion channel incorporated in an artificial lipid bilayer. Hidden conformational changes were observed, which strongly suggests that multiple intermediate conformation states are involved in gramicidin ion channel dynamics. (C) 2004 American Institute of Physics.

引用

页码：1792 / 1794

页数：3

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