B16LS9 melanoma cells spread to the liver from the murine ocular posterior compartment (PC)

被引:38
作者
Diaz, CE
Rusciano, D
Dithmar, S
Grossniklaus, HE
机构
[1] Emory Univ, Sch Med, Dept Ophthalmol, Atlanta, GA 30322 USA
[2] Friedrich Miescher Inst, CH-4002 Basel, Switzerland
关键词
animal model; ciliary body; choroid; liver; metastasis; mouse; melanoma;
D O I
10.1076/ceyr.18.2.125.5380
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose. To create a murine ocular melanoma model that consistently metastasizes to the liver. Methods. Twelve-week-old C57BL6 mice (n=10) were inoculated in the posterior compartment (PC) of one eye with 5 x 10(5) tissue culture B16LS9 melanoma cells. The inoculated eyes were enucleated at two weeks and the mice were sacrificed with necropsies performed at four weeks post-inoculation. Results. Melanoma grew and was confined to the eyes of all 10 mice. The melanoma hematogenously spread to the lungs in 9 of 10 mice and the liver in 8 of 10 mice. There was a positive correlation between pulmonary and liver metastasis (r = 0.94). Conclusions. B16LS9 melanoma cells consistently hematogenously spread to the liver when implanted into C57BL6 mice eyes. The value of this model is unknown at this time since it is not known if the intrahepatic melanoma is capable of growing and killing the host.
引用
收藏
页码:125 / 129
页数:5
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