Flow injection immunosensing of polycyclic aromatic hydrocarbons with a quartz crystal microbalance

被引：43

作者：

Liu, M

Li, QX

Rechnitz, GA ^{[1
]}

机构：

[1] Univ Hawaii, Dept Chem, Hawaii Biosensor Lab, Honolulu, HI 96822 USA

[2] Univ Hawaii, Dept Environm Biochem, Honolulu, HI 96822 USA

来源：

ANALYTICA CHIMICA ACTA | 1999年 / 387卷 / 01期

基金：

美国国家科学基金会;

关键词：

immunosensors; piezoelectric sensors; self-assembled monolayer; monoclonal antibody 10c10; polycyclic aromatic hydrocarbons;

D O I：

10.1016/S0003-2670(99)00030-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A piezoelectric immunosensor has been developed for the detection of various polycyclic aromatic hydrocarbons (PAHs). The antigen (benzo[a]pyrene-BSA conjugate, BaP-BSA) was immobilized through thioctic acid on gold coated quartz crystals, with a basic resonant frequency of 10 MHz. The monoclonal antibody 10c10 (mAb10c10), which specifically recognizes benzo[a]pyrene (BaP), was detected through frequency decreases (mass increase due to the immunoreaction). Determination of BaP was performed in the flow injection system by using a competitive pattern, in which BaP reacted with the bound mAb10c10 causing frequency increases. A nanomolar level of BaP can be detected with this immunosensor. The mAb10c10 modified quartz crystal cross-reacts with pyrene and naphthalene. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：29 / 38

页数：10

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