Study on an antimicrobial protein produced by Paenibacillus polymyxa JS']JSa-9 isolated from soil

被引：14

作者：

Deng, Yang ^{[1
]}

Lu, Zhaoxin ^{[1
]}

Lu, Fengxia ^{[1
]}

Wang, Yu ^{[1
]}

Bie, Xiaomei ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Food Sci & Technol, Key Lab Food Proc & Qual Control, Minist Agr China, Nanjing 210095, Peoples R China

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2011年 / 27卷 / 08期

关键词：

Antimicrobial glycoprotein; Paenibacillus polymyxa; Purification; Amino acid composition; N-terminal sequence; BACILLUS-SUBTILIS; PEPTIDE ANTIBIOTICS; SYSTEM;

D O I：

10.1007/s11274-010-0638-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Paenibacillus polymyxa strain JSa-9, a soil isolate showed in vitro inhibitory effects on several pathogens. A protein of about 71.9 kDa, isolated from a culture of JSa-9, exhibited broad-spectrum antibacterial and antifungal activities. The purification procedure consisted of ion-exchange chromatography on DEAE-Sephacel and Sephadex G-100 column chromatography. The purified protein was characterized to be a glycoprotein, which contained 53% of amino acids and 28% of carbohydrate. Its N-terminal sequence was determined as KCATIPVVIKHL and the amino acid composition showed no significant homology with any known antagonistic proteins from P. polymyxa. Because of the excellent antimicrobial activity, this protein may be a good prospect for food preservation and plant disease control.

引用

页码：1803 / 1807

页数：5

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