Circulating Cell-Derived Microparticles in Severe Preeclampsia and in Fetal Growth Restriction

被引:23
作者
Alijotas-Reig, Jaume [1 ,2 ]
Palacio-Garcia, Carles [3 ]
Farran-Codina, Immaculada [4 ]
Ruiz-Romance, Mar [4 ]
Llurba, Elisa [1 ,4 ]
Vilardell-Tarres, Miquel [2 ]
机构
[1] Vall dHebron Univ Hosp, Dept Internal Med 1, Syst Autoimmune Dis Unit, Barcelona, Spain
[2] Univ Autonoma Barcelona, Fac Med, Dept Med, Barcelona 08035, Spain
[3] Univ Autonoma Barcelona, Vall dHebron Univ Hosp, Haematol Serv, Flow Cytometry Unit, Barcelona 08035, Spain
[4] Univ Autonoma Barcelona, Vall dHebron Univ Hosp, Dept Obstet, High Obstet Risk Unit, Barcelona 08035, Spain
关键词
Antiphospholipid antibodies; cell-derived microparticles; fetal growth restriction; severe preeclampsia; HELLP syndrome; SYSTEMIC-LUPUS-ERYTHEMATOSUS; ENDOTHELIAL MICROPARTICLES; ANTIPHOSPHOLIPID SYNDROME; RECURRENT MISCARRIAGE; COMPLEMENT ACTIVATION; NORMAL-PREGNANCY; THROMBOPHILIA; DYSFUNCTION; GENERATION; REJECTION;
D O I
10.1111/j.1600-0897.2011.01072.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Problem The behavior of the circulating microparticles (cMP) in severe preeclampsia (PE) and fetal growth restriction (FGR) is disputed. Method of study Non-matched case-control study. Seventy cases of severe PE /HELLP /FGR were compared to 38 healthy pregnant women. Twenty healthy non-pregnant women acted as a control. cMP were analyzed using flow cytometry. Results are given as total (annexin-A5-ANXA5+), platelet (CD41+), leukocyte (CD45+), endothelial (CD144+CD31+//CD41)), and CD41-negative cMP/mu L of plasma. Antiphospholipid antibodies (aPL) were analyzed through usual methods. Results Platelet and endothelial cMP increased in healthy pregnant women. PE whole group (PE +/- FGR) showed an increase in endothelial and CD41negative, but not in platelet-derived, cMP. Comparing PE whole group versus healthy pregnant, we found cMP levels of endothelial and CD41) had increased. The cMP results obtained in PE group were similar to those of the PE whole group. Comparing PE group to isolated FGR, significant CD41-negative cMP increase was found in PE. According to its aPL positivity, a trend to decrease in leukocyte and endothelial-derived cMP was found in PE group. Conclusion Normal pregnancy is accompanied by endothelial and platelet cell activation. Endothelial cell activation has been shown in PE but not in isolated FGR. In PE, aPL may contribute to endothelial and possibly to leukocyte cell activation.
引用
收藏
页码:140 / 151
页数:12
相关论文
共 46 条
[1]  
ACOG, 2002, OBSTET GYNECOL, V99, P869
[2]   Recurrent miscarriage and inherited thrombophilia:: diagnostic work-out and therapeutic management [J].
Alijotas-Reig, J ;
Ferrer-Raventós, JC .
MEDICINA CLINICA, 2005, 125 (16) :626-631
[3]  
Alijotas-Reig J, 2009, 7 M EUR FOR ANT ANT
[4]   Is Obstetric Antiphospholipid Syndrome a Primary Nonthrombotic, Proinflammatory, Complement-Mediated Disorder Related to Antiphospholipid Antibodies? [J].
Alijotas-Reig, Jaume ;
Vilardell-Tarres, Miquel .
OBSTETRICAL & GYNECOLOGICAL SURVEY, 2010, 65 (01) :39-45
[5]  
[Anonymous], 2000, AM J OBSTET GYNECOL, V188, pS1
[6]   Cell-derived microparticles and complement activation in preeclampsia versus normal pregnancy [J].
Biro, E. ;
Lok, C. A. R. ;
Hack, C. E. ;
van der Post, J. A. M. ;
Schaap, M. C. L. ;
Sturk, A. ;
Nieuwland, R. .
PLACENTA, 2007, 28 (8-9) :928-935
[7]   INDUCTION OF ENDOTHELIAL-CELL TISSUE FACTOR ACTIVITY BY SERA FROM PATIENTS WITH ANTIPHOSPHOLIPID SYNDROME - A POSSIBLE MECHANISM OF THROMBOSIS [J].
BRANCH, DW ;
RODGERS, GM .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1993, 168 (01) :206-210
[8]  
Brandt JT, 1995, THROMB HAEMOSTASIS, V126, P555
[9]   Circulating microparticles: a marker of procoagulant state in normal pregnancy and pregnancy complicated by preeclampsia or intrauterine growth restriction [J].
Bretelle, F ;
Sabatier, F ;
Desprez, D ;
Camoin, L ;
Grunebaum, L ;
Combes, V ;
D'Ercole, C ;
Dignat-George, F .
THROMBOSIS AND HAEMOSTASIS, 2003, 89 (03) :486-492
[10]   Prevalence of circulating procoagulant microparticles in women with recurrent miscarriage: a case-controlled study [J].
Carp, H ;
Dardik, R ;
Lubetsky, A ;
Salomon, O ;
Eskaraev, R ;
Rosenthal, E ;
Inbal, A .
HUMAN REPRODUCTION, 2004, 19 (01) :191-195