In vivo photocycle of the Euglena gracilis photoreceptor

被引：24

作者：

Barsanti, L

Passarelli, V

Walne, PL

Gualtieri, P

机构：

[1] CNR, IST BIOFIS, I-56127 PISA, ITALY

[2] UNIV TENNESSEE, DEPT BOT, KNOXVILLE, TN 37996 USA

来源：

BIOPHYSICAL JOURNAL | 1997年 / 72卷 / 02期

关键词：

D O I：

10.1016/S0006-3495(97)78694-6

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We present the light-induced photocycle of the paraflagellar swelling of Euglena gracilis. The kinetics of this process was reconstructed by sampling its fluorescence emission and switching the excitation light from 365 nm to 436 nm. Stable intermediates in the photocycle were manifested. The measured millisecond resolution kinetics best fits a Michaelis-Menten equation. The data provide strong evidence that the paraflagellar swelling, a three-dimensional natural crystal of a light-detecting protein, is the true Euglena photoreceptor.

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页码：545 / 553

页数：9

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