Introduction of enterostatin (VPDPR) and a related sequence into soybean proglycinin A1aB1b subunit by site-directed mutagenesis

被引：61

作者：

Takenaka, Y ^{[1
]}

Utsumi, S ^{[1
]}

Yoshikawa, M ^{[1
]}

机构：

[1] Kyoto Univ, Food Sci Res Inst, Kyoto 6110011, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 12期

关键词：

anoretic activity; enterostatin; glycinin; hypocholesterolemic activity; soybean;

D O I：

10.1271/bbb.64.2731

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Enterostatin (VPDPR), having anoretic and hypocholesterolemic activities, and its homologue LPYPR, a hypocholesterolemic peptide found in the glycinin A5A4B3 subunit, were introduced into the corresponding site (TNGPQ) of the proglycinin A1aB1b subunit by site-directed mutagenesis. Modified proglycinins were expressed in E. coli and recovered from the insoluble fraction. VPDPR and LPYPR were released by the action of chymotrypsin and trypsin as expected. The overall yields of purified VPDPR and LPYPR were 40% and 62%, respectively. © 2000, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：2731 / 2733

页数：3

共 12 条

[1]

THE GLYCININ-GY3 GENE FROM SOYBEAN [J].