Basic methods in histopathology of joint tissues

被引：359

作者：

Schmitz, N. ^{[2
]}

Laverty, S. ^{[3
]}

Kraus, V. B. ^{[4
]}

Aigner, T. ^{[1
]}

机构：

[1] Med Ctr Coburg, Inst Pathol, D-96540 Coburg, Germany

[2] Univ Leipzig, Inst Pathol, D-04103 Leipzig, Germany

[3] Duke Univ, Med Ctr, Durham, NC 27710 USA

[4] Univ Montreal, Fac Med Vet, Dept Clin Sci, St Hyacinthe, PQ J2S 7C6, Canada

来源：

OSTEOARTHRITIS AND CARTILAGE | 2010年 / 18卷

关键词：

Cartilage; Osteoarthritis histochemistry; Toluidine blue; Safranin O; Picrosirius red Goldner's trichrome stain hematoxylin-eosine; PROTEOGLYCAN; MICROSCOPY; CARTILAGE; EDTA;

D O I：

10.1016/j.joca.2010.05.026

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

Histological and histochemical methods are important tools in the evaluation of joint tissue samples for degenerative joint diseases, both in humans and in animal models In this respect. standardized, simple, and reliable techniques are mandatory This chapter describes five basic staining procedures appropriate for macroscopic (Indian ink) and histologic (HE/hematoxylin - eosin) visualization and scoring of cartilage proteoglycan and collagen content (toluidine blue/safranin O and picrosirius red/Goldner's trichrome) (C) 2010 Osteoarthritis Research Society International Published by Elsevier Ltd. All rights reserved

引用

页码：S113 / S116

页数：4

共 7 条

[1]

Effect of bone decalcification procedures on DNA in situ hybridization and comparative genomic hybridization: EDTA is highly preferable to a routinely used acid decalcifier [J].