Simultaneous measurements of intracellular cAMP and L-type Ca2+ current in single frog ventricular myocytes

被引:51
作者
Goaillard, JM
Vincent, P
Fischmeister, R
机构
[1] Univ Paris Sud, Fac Pharm, INSERM, U446,Lab Cardiol Cellulaire & Mol, F-92296 Chatenay Malabry, France
[2] Univ Paris 06, Lab Neurobiol Cellulaire, CNRS, UMR 7624, F-75005 Paris, France
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2001年 / 530卷 / 01期
关键词
D O I
10.1111/j.1469-7793.2001.0079m.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. The cAMP fluorescent probe FlCRhR was used to monitor changes in intracellular cAMP concentration ([cAMP](i)) in isolated frog ventricular rnyocytes. The probe was introduced into the cell through a patch pipette which allowed simultaneous recording of the whole-cell L-type Ca2+ current (I-Ca). Ratiometric imaging was used to monitor [cAMP](i) changes in response to the beta -adrenergic agonist isoprenaline (ISO) or to the direct adenylyl cyclase activator forskolin (FSK). 2. FlCRhR fluorescence was distributed in the cytosol in a striated pattern, with high fluorescence in the I-bands and low fluorescence in the A-bands. This pattern of distribution was mimicked by fluorescein dextran, another high molecular weight fluorescent molecule, and was therefore likely to be due to anisotropic diffusion of the probe in the cytosol due to the hindrance generated by sarcomeric proteins in the A-bands. 3. Introduction of FlCRhR into the cell induced a small similar to 70% stimulatory effect on basal I-Ca, attenuating about 2-fold a subsequent response of I-Ca to 1-10 muM ISO (from 400 to 200%). 4. Brief (10 s) application of a saturating concentration of ISO (1-20 muM) to the cell induced a transient increase in both I-Ca and [cAMP](i). However, the [cAMP](i) transient was similar to2-fold shorter in duration than the I-Ca transient, i.e. I-Ca was still strongly enhanced when [cAMP](i) had already returned to control level. This indicates that hydrolysis of cAMP by phosphodiesterases is not a rate limiting step in the recovery of I-Ca from ISO stimulation. 5. When the application of ISO was maintained, I-Ca and [cAMP](i) responses followed a similar time course, with a half-maximal response at similar to 60 s. This suggests that activation of Ca2+ channels by cAMP-dependent protein kinase occurs on a much faster time scale than the rise in [cAMP](i). 6. When the cells were exposed to FSK (13 muM), both responses of I-Ca and [cAMP](i) were similar to2-fold slower than with ISO. This demonstrates that the slower response of I-Ca to FSK is due to a slower rise in [cAMP](i) rather than to some inhibitory effect of FSK on I-Ca or to a direct or priming effect of the stimulatory G protein G(s) on Ca2+ channels. 7. Simultaneous measurements of [cAMP](i) and I-Ca changes in intact cardiac myocytes opens the way to dissect the temporal sequence of events in the cAMP cascade mediating the response of the heart to a large number of hormones and inotropic agents.
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页码:79 / 91
页数:13
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