Crystallization and preliminary X-ray analysis of the major peanut allergen Ara h 1 core region

被引：10

作者：

Cabanos, Cerrone ^{[1
]}

Urabe, Hiroyuki ^{[1
]}

Masuda, Taro ^{[1
]}

Tandang-Silvas, Mary Rose ^{[1
]}

Utsumi, Shigeru ^{[1
]}

Mikami, Bunzo ^{[2
]}

Maruyama, Nobuyuki ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Lab Food Qual Design & Dev, Kyoto, Japan

[2] Kyoto Univ, Grad Sch Agr, Lab Appl Struct Biol, Kyoto, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2010年 / 66卷

关键词：

peanut allergens; Ara h 1; RECOMBINANT;

D O I：

10.1107/S1744309110029040

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Peanuts contain some of the most potent food allergens known to date. Ara h 1 is one of the three major peanut allergens. As a first step towards three-dimensional structure elucidation, recombinant Ara h 1 core region was cloned, expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using 0.1 M sodium citrate pH 5.6, 0.1 M NaCl, 15% PEG 400 as precipitant. The crystals diffracted to 2.25 A resolution using synchrotron radiation and belonged to the monoclinic space group C2, with unit-cell parameters a = 156.521, b = 88.991, c = 158.971 A, beta = 107.144 degrees. Data were collected at the BL-38B1 station of SPring-8 (Hyogo, Japan).

引用

页码：1071 / 1073

页数：3

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